megatomi.com for Dummies
megatomi.com for Dummies
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Our new Obvious+ tissue clearing procedure is the only real method that delipidates samples with no adjust in morphology and with negligible impact on structural integrity.
Megatome is really a vibrating microtome built to portion a broad selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome allows significant-throughput tissue sectioning with uniform area profile, and minimal tissue harm and data reduction.
Antibodies may well acquire months to diffuse by only some millimeters of tissue, that has a steep labeling gradient from area to Main.
Megatome is suitable for precision: the blade vibrates at an increased frequency and bigger amplitude selection than other microtomes, and incorporates a exclusive deflection Regulate system.
SE employs a rotational electrical area to disperse extremely electromobile molecules (such as antibodies or surfactant micelles) all over a porous sample devoid of detrimental electrically charged structures within the tissue. This allows two-4 working day clearing of intact organs,
Our preformulated EasyIndex Remedy raises and homogenize the refractive index of delipidated tissue samples, rendering them fully clear. This permits mild penetration into the sample and makes sure the acquisition of significant-resolution, in-emphasis image data.
Megatome is often a novel microtome that permits for top-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with small tissue injury and knowledge megatomi.com reduction.
It’s time for you to up grade your microtome to Megatome. With precise higher-frequency slicing for an unmatched number of sample measurements and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for various purposes.
The SmartSPIM mild sheet microscope provides unparalleled resolution and acquisition pace throughout intact tissue volumes, even though its modular style means that you can improve and customise person factors.
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Possibly download a release or develop a distribution zip as outlined above. Unzip the archive to your desired area.
Leverage the Very clear+ tissue clearing strategy, in conjunction with eFLASH and patented stochastic electrotransport technologies, to quickly very clear and label total organs. Critical highlights and features consist of:
Our novel Defend tissue preservation strategy varieties intramolecular bonds applying polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.